ARACUS Amino Acid Analyzer from Membrapure

ARACUS Amino Acid Analyzer from Membrapure - innovative technology combined with the classical method.

The amino acid analyzer ARACUS with its modular concept is the ideal instrument for the analysis of amino acids in research, quality control and in the clinical laboratory. It combines innovative technologies with the classical routine analysis of amino acids by the post- column derivatisation with ninhydrin. This produces results with the highest precision, reproducibility and accuracy.

The setup of the instrument allows the continuous analysis of up to 96 samples or 192 samples. The samples are stored in the cooled autosampler and the samples are injected without sample loss. Washing the injection valve and syringe pump guarantees zero cross-contamination.

As a third option the ARACUS instrument is also available with a manual injection system.

The eluent rack contains 6 glass bottles. The fluid volume of each bottle is actively monitored during operation. Eluents, ninhydrin and washing reagent are selectively chosen through buffer selection valves and degassed in micro vacuum chambers. The innovative technology of the pump allows the simultaneous operation of two fluid lines (eluent side and reagent side), which guarantees constant mixing.

The maintenance free photometers detect the separated amino acids at 570 nm and 440 nm and the signals registered by the aminoPeak software.

Innovative chemistry

The concept of the ready-to-use eluent and reagent kits allows the reproducible analysis of the amino acid samples. Each kit is produced under standardized procedures, tested and certified before shipping it to the customer. Depending on the application, each kit contains the necessary amount for either 500 or 1000 analysis runs.

Operation Control

ARACUS is operated with the user friendly, self-explanatory software iControl. Optimised separation programs are correlated with each sample for the analysis. Correlation with new samples can be done whilst the instrument is analyzing a sample. The current status of important instrument parameters is shown as well as the fluid volumes of each eluent and reagent bottle. As soon as a minimal value is reached, different alarm levels inform the operator. All operation parameters and events of each analysis are documented in a log-File.

Data Acquisition and Handling

The acquisition and analysis software aminoPeak records simultaneously two analogue channels (570 nm, 440 nm). Chromatograms are shown on-line. Internal data bank allows fast peak identification. Quantitative calculations are performed with internal or external standards, dilution and/or multiplication factors. Several analysis runs can be compared using compiler programs. The result of each analysis is documented in an individual report.